ABSTRACT
As an important component of the tumor microenvironment, cancer-associated fibroblasts (CAFs) secrete energy metabolites to supply energy for tumor progression. Abnormal regulation of long noncoding RNAs (lncRNAs) is thought to contribute to glucose metabolism, but the role of lncRNAs in glycolysis in oral CAFs has not been systematically examined. In the present study, by using RNA sequencing and bioinformatics analysis, we analyzed the lncRNA/mRNA profiles of normal fibroblasts (NFs) derived from normal tissues and CAFs derived from patients with oral squamous cell carcinoma (OSCC). LncRNA H19 was identified as a key lncRNA in oral CAFs and was synchronously upregulated in both oral cancer cell lines and CAFs. Using small interfering RNA (siRNA) strategies, we determined that lncRNA H19 knockdown affected proliferation, migration, and glycolysis in oral CAFs. We found that knockdown of lncRNA H19 by siRNA suppressed the MAPK signaling pathway, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) and miR-675-5p. Furthermore, the lncRNA H19/miR-675-5p/PFKFB3 axis was involved in promoting the glycolysis pathway in oral CAFs, as demonstrated by a luciferase reporter system assay and treatment with a miRNA-specific inhibitor. Our study presents a new way to understand glucose metabolism in oral CAFs, theoretically providing a novel biomarker for OSCC molecular diagnosis and a new target for antitumor therapy.
Subject(s)
Humans , Cancer-Associated Fibroblasts/metabolism , Carcinoma, Squamous Cell/genetics , Cell Line, Tumor , Cell Proliferation , Gene Expression Regulation, Neoplastic , Glycolysis , Head and Neck Neoplasms , MicroRNAs/metabolism , Mouth Neoplasms/genetics , Phosphofructokinase-2/genetics , RNA, Long Noncoding/genetics , Signal Transduction , Tumor MicroenvironmentABSTRACT
<p><b>OBJECTIVE</b>To evaluate the anesthetic effects and safety of Gow-Gates technique of inferior alveolar nerve block in impacted mandibular third molar extraction.</p><p><b>METHODS</b>A split-mouth study was designed. The bilateral impacted mandibular third molar of 32 participants were divided into Gow-Gates technique of inferior alveolar nerve block (Gow-Gates group) and conventional technique of inferior alveolar nerve block (conventional group) randomly with third molar extracted. The anesthetic effects and adverse events were recorded.</p><p><b>RESULTS</b>All the participants completed the research. The anesthetic success rate was 96.9% in Gow-Gates group and 90.6% in conventional group with no statistical difference ( P= 0.317); but when comparing the anesthesia grade, Gow-Gates group had a 96.9% of grade A and B, and conventional group had a rate of 78.1% (P = 0.034). And the Gow-Gates group had a much lower withdrawn bleeding than conventional group (P = 0.025). Two groups had no hematoma.</p><p><b>CONCLUSION</b>Gow-Gates technique had a reliable anesthesia effects and safety in impacted mandibular third molar extraction and could be chosen as a candidate for the conventional inferior alveolar nerve block.</p>
Subject(s)
Female , Humans , Anesthesia, Dental , Anesthetics , Injections , Mandible , Mandibular Nerve , Molar , Molar, Third , Nerve Block , Tooth ExtractionABSTRACT
<p><b>OBJECTIVE</b>The purpose of this study was to investigate the indication and therapeutic effects of Pingyangmycin injection as a primary therapy of lymphangiomas in oral, maxillofacial and cervical region.</p><p><b>METHODS</b>A total of 195 patients (106 males and 89 females) with lymphangiomas in oral and maxillofacial regions were treated in the affiliated dental hospital of Sichuan University from May 1990 to December 2000. The patients' ages ranged from 0.5 to 46 years. The tongue was the most commonly involved site, followed by the cheek and the neck. The 200 lymphangiomas (5 patients had 2 lymphangiomas in different sites) underwent the therapy of Pingyangmycin, which was injected as with 1 mg/ml in saline. The total dose of Pingyangmycin ranged from 5 mg to 70 mg and 5 to 58 times, 1 time per 2-4 weeks.</p><p><b>RESULTS</b>The curative rate of cystic-type lymphangiomas was the highest. Of the 51 cystic lymphangiomas, 110 capillary lymphangiomas, 18 cavernous lymphangiomas and 21 combinations of capillary and cavenous lymphangiomas, the curative rates were respectively 100% (51), 46.36% (51), 16.16% (3) and 19.05% (4), which showed a significant therapeutic effect, respectively. And 40(78.43%), 19(17.27%), 2(11.11%) and 0(0%) of them completely disappeared. There was no serious side effect with Pingyangmycin-injection treatment, such as pulmonary fibrosis.</p><p><b>CONCLUSION</b>The treatment of injection of Pingyangmycin is a selective primary method of lymphangiomas, which can reduce the size of lymphangiomas, and make them completely disappeared.</p>
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Antibiotics, Antineoplastic , Bleomycin , Dexamethasone , Drug Administration Schedule , Head and Neck Neoplasms , Drug Therapy , Injections, Intralesional , Lymphangioma , Drug Therapy , Mouth Neoplasms , Drug Therapy , Tongue Neoplasms , Drug TherapyABSTRACT
0.05), except PYM-AMS of 1.130 mg/ml and PYM of 300 ?g/ml at 24 h.In the same group and at the same exposure time,higher dose of the corresponding agents showed higher growth inhibition ratio(P0.05).At the same doses 48 h exposure of PYM-AMS or PYM gave higher apoptosis rate than 24 h exposure(P
ABSTRACT
Objective: To evaluate the effect of pingyangmycin-albumin microspheres (PYM-AMS) on the modality of endothelial cells. Methods:In the in vitro study blood vessle endothelial cells of ECV 304 cell line were exposed to PYM-AMS(containing PYM at 150 ?g/ml),AMS and PYM(150 ?g/ml) for 24,48,72 and 96 h respectively. In the in vivo study,24 Japanese white rabbits were divided into 4 groups using randomized block design. PYM+9 g/L NaCl,PYM+soyabean oil, PYM-AMS+soyabean oil(PYM at 5 mg/ml) were injected into the central auricular arteries of the animals(0.26 ml/per ear), then these vessels were examined histologically 2, 7, 14, 21 days after injection respectively. The mophorlogy of the cells was observed by light microscope and electron microscope.Results:In vitro, a great part of cells were swollen and cell number decreased in PYM and PYM-AMS group. But there was no change in AMS group.In vivo, the endothelial cells had no significant changes in PYM+9 g/L NaCl group.In PYM+soyabean oil group, at the 2nd day, the endothelial cells were a little bit swollen. At the 7th day, some endothelial cells were dropped off. At the 21st day, a few of endothelial cells were proliferative. In PYM-AMS group, at the 2nd day, the endothelial cells were a little bit swollen and small vessels were embolized by PYM-AMS. At the 7th day, the endothelial cells were swollen. At the 14th day, the endothelial cells were proliferative and the wall of the central auricular artery had more layers. The lumen of the central artery became smaller, while the surface of PYM-AMS was absorbed. At the 21st day, the wall of the central auricular artery was proliferative and the artery became sclerostenosed. The PYM-AMS was obviously absorbed, while the wall of small vein was proliferative, too. Conclusion:PYM-AMS and PYM may injure blood vessel endothelial cells, the effect of PYM-AMS is more obvious than pingyangmycin.